Technical Data
C2415-31D
CD62L (gp90 MEL, hLHRc, L Selectin, L-Selectin, LECAM-1, Lymph Node Homing Receptor, LNHR, Lymphocyte Adhesion Molecule 1, LYAM1, PLNHR, Selectin L, SELL, TQ1) (PE)
Description:
CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains.1, 2 It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes.1, 2 In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils.3-7 This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation.1, 2, 7-9 Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.10-13 The monoclonal antibody blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.1, 2, 7-9

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, in vivo and in vitro blocking of adhesion and most Cytotoxicity studies. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a,kMEL-14Purified
SizeStorageShippingSourceHost
100ug4°C Do not freezeBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
C3H/eb cloned mouse B lymphoma 38C-13.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes mouse CD62L/L-selectin, Mr 90kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gallatin, W.M., I.L. Weissman, and E.C. Butcher. 1983. Nature 304:30. 2. Siegelman, M.H., I.C. Cheng,, I.L. Weissman, and E.K. Wakeland. 1990. Cell 61:611. 3. Reichert, R.A., I.L. Weissman, and E.C. Butcher. 1986. J. Immunol. 136:3521. 4. Reichert, R.A., I.L. Weissman, and E.C. Butcher. 1986. J. Immunol. 136:3529. 5. Reichert, R.A., L. Jerabek, W.M. Gallatin, E.C. Butcher, and I.L. Weissman. 1986. J. Immunol. 136:3535. 6. Iwabuchi, K., J. Ohgama, K. Ogasawara, C. Iwabuchi, I. Negishi, R.A. Good, and K. Onoe. 1991. Immunobiology 182:161. 7. Lewinsohn, D.M., R.F. bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313. 8. Pizcueta, P., and F.W. Luscinskas. 1994. Am. J. Pathol. 145:461. 9. Ley, K., D.C. Bullard, M.L. Arbones, R. Bosse, D. Vestweber, T.F. Tedder, and A.L. Beaudet. 1995. J. Exp. Med. 181:669. 10. Jung, T.M., W.M. Gallatin, I.L. Weissman, and M.O. Dailey. 1988. J. Immunol. 141:4110. 11. Kishimoto, T.K., M.A. Jutila, E.L. berg, and E.C. Butcher. 1989. Science 245:1238. 12. Mobley, J.L., and M.O. Dailey. 1992. J. Immunol. 148:2348. 13. Sprent, J., and D.F. Tough. 1994. Science 265:1395.