Technical Data
C2421-03
CD66b (CEACAM-8, CEA-related cell adhesion molecule 8, CGM6, CEA gene family member 6, NCA-95, Nonspecific crossreacting antigen 95)
Description:
CD66b is a 95-100kD GPI anchored molecule and is expressed strongly by mature granulocytes and metamyelocytes but weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1:50-1:100. Use 10ul labels 5x10e5 cells or 100ul whole blood.
Immunohistochemistry (Frozen): 1:25-1:100.
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1903-13A: IgG, F(ab)2, X-Adsorbed (PE) Pab: Gt xMo
I1904-19C: IgG, F(ab)2 (HRP) Pab Gt xMo

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse MOPC 315-43 myeloma cell line.

Recommended Negative Controls:
I1904-78R: IgG1 Murine Negative Control

Recommended Positive Control Tissue:
Bone Marrow

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H1637Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
CML leukocytes
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.09% sodium azide.
Specificity:
Recognizes the CGM6 gene product of the carcinoembryonic gene family. CD66b is expressed strongly by mature granulocytes and metamyelocytes but weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mannoni, P. et al. (1982) Monoclonal antibodies against human granulocytes and myeloid differentiation. Hum. Immunol. 5: 309-323. 2. Ionita, M.G. et al. (2010) High Neutrophil Numbers in Human Carotid Atherosclerotic Plaques Are Associated With Characteristics of Rupture-Prone Lesions. Arterioscler Thromb Vasc Biol. Jul 1. 3. Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism. J. Leukoc. Biol. 87: 905-14. 4. Feuk-Lagerstedt, E. et al. (1999) Identification of CD66a and CD66b as the major galectin-3 receptor candidates in human neutrophils. J Immunol. 163: 5592-8. 5. Jankowski, A. et al. (2002) Determinants of the phagosomal pH in neutrophils. J Biol Chem. 277: 6059-66. 6. Jinnouchi, A. et al. (2005) Local anesthetics inhibit priming of neutrophils by lipopolysaccharide for enhanced release of superoxide: suppression of cytochrome b558 expression by disparate mechanisms. J Leukoc Biol. 78: 1356-65. 7. Thickett, D.R. et al. (2002) A role for vascular endothelial growth factor in acute and resolving lung injury. Am J Respir Crit Care Med. 166: 1332-7. 8. Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slides.
J Immunol Methods. 313: 96-109. 9. Ottonello, L. et al. (1999) Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 93: 3505-11. 10. Ottonello, L. et al. (2000) Monoclonal Lym-1 antibody-targeted lysis of B lymphoma cells by neutrophils. Evidence for two mechanisms of FcgammaRII-dependent cytolysis. J Leukoc Biol. 68: 662-8. 11. Moraes, T.J. et al. (2006) Abnormalities in the pulmonary innate immune system in cystic fibrosis. Am J Respir Cell Mol Biol. 34: 364-74. 12. Nijhuis, J. et al. (2009) Neutrophil Activation in Morbid Obesity, Chronic Activation of Acute Inflammation Obesity (Silver Spring). 17: 2014-8. 13. Pliyev, B.K. and Menshikov, M.Y. (2010) Release of the soluble urokinase-type plasminogen activator receptor (suPAR) by activated neutrophils in rheumatoid arthritis. Inflammation. 33:1-9. 14. Binet, F. and Girard, D. (2008) Novel human neutrophil agonistic properties of arsenic trioxide: involvement of p38 mitogen-activated protein kinase and/or c-jun NH2-terminal MAPK but not extracellular signal-regulated kinases-1/2. J Leukoc Biol. 84: 1613-22. 15. Rosenkranz, A.R. et al. (1999) Novel C5-dependent mechanism of neutrophil stimulation by bioincompatible dialyzer membranes. J Am Soc Nephrol. 10: 128-35. 16. Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism. J Leukoc Biol. 87: 905-14. 17. Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slides J Immunol Methods. 313: 96-109.