Technical Data
C2423-02
CD68 (MACROSIALIN, DKFZp686M18236, Macrophage antigen, SCARD1, Scavenger receptor class D member1)
Description:
C2423-02 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. C2423-02 reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:200-1:400 for 20 minutes at RT. For staining of formalin-fixed tissues, digest sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
Optimal dilution determined by the researcher.

Positive Control:
Tonsil, lymph node or spleen

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3F103Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Subcellular fraction of human alveolar macrophages. Cellular Localization: Cytoplasmic
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes the human glycoprotein of 110kD, which is identified as CD68. Species Crossreactivity: monkey, feline and rat. Does not react with porcine, canine or chicken
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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