		Technical Data
C2423-05G	CD68 (MACROSIALIN, DKFZp686M18236, Macrophage antigen, SCARD1, Scavenger receptor class D member1)

Description:
CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization.
CD68 is a 110kD transmembrane (Type I) glycoprotein consisting of a polypeptide of 37.4k D (354 amino acids) predicted from the cDNA sequence that is highly expressed by human monocytes and tissue macrophages. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): Neat. Proteolytic treatment is required. High temperature antigen unmasking may improve the staining. Incubation 60 minutes at RT.
Immunohistochemistry (Frozen): Neat. Acetone fixed.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	8J879	Ascites

Size		Storage	Shipping	Source	Host
6ml		-20°C	Blue Ice	Human	Mouse

Concentration:

Not Determined

Immunogen:

Subcellular fraction of human alveolar macrophages.

Purity:

Ascites

Form

Supplied as a liquid in PBS, 0.1% sodium azide

Specificity:

Recognizes human CD68. Specific to macrophages in a wide variety of human tissues. Reacts with myeloid precursors and peripheral blood granulocytes and also stains a cell population known as Plasmacytoid T cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Pulford KA et al. KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol 42:414-21 (1989).