Technical Data
C2425-01M
CD70 (Tnfsf7, CD27 Ligand) (Biotin)
Description:
Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Functional Application:
Antibody inhibits T cell proliferation induced by dendritic cells.

Recommended Dilutions:
Flow Cytometry: 10ug/ml. Use 80ul to label 5x10e5 Raji cells.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Recommended Negative Control:
I1904-78R2: IgG1 Murine Negative Control (Biotin)

Flow Cytometry Protocol:
1. 5x10e5 cultured Raji cells were washed, then incubated 45 minutes on ice with 80ul of C2425-01M at 10ug/ml.
2. Cells were then washed twice and incubated for 45 minutes with S7973-88P: Streptavidin (PE).
3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained with a mean shift of 1.32 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration.
5. Binding was blocked when pre-incubated for 10 minutes with 20ul of 0.5mg/ml of anti-CD70 (C2425-02).

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG17H173Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human WM-1 (Waldenström's macroglobulinemia) cell line.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Specificity:
Recognizes the human CD70 molecule of ~30kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hintzen, R.Q., et al., J. Immunol. 152: 1762-1773 (1994). 2. Bowman, M.R., et al., J. Immunol. 152: 1756-1761 (1994). 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, p. 1137-1138 (1995). 4. Agematsu, K., et al., J. Immunol. 154: 3627-3635 (1995). 5. Iwamoto, S., et al., J. Leuk. Biol. 78: 383-392 (2005). 6. Wischhusen, J., et al., Cancer Res. 62: 2592-2599 (2002). 7. Adams, P.J., et al., British J. Cancer 95: 298-306 (2006). 8. Muller, C., et al., Intl. Immunol. 22: 289-298 (2010).