Technical Data
C2425-01P
CD70 (Tnfsf7, CD27 Ligand) (PE)
Description:
Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50 (or use 1.6ul of concentrated stock per 5x10e5 cell test).
Optimal dilutions to be determined by the researcher.

FACS Protocol:
1. Five x 10e5 cultured Raji human tumor cells were washed and incubated for 45 minutes on ice with 80ul of C2425-01P at a 1:50 dilution (10ug/ml).
2. Cells were washed three times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a I1904-79D IgG1 Murine Negative Control (PE) at a similar concentration.
4. Binding was blocked when pre-incubated for 10 minutes with 20ul of 0.5mg/ml of C2425-01L.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG17H173Affinity Purified
SizeStorageShippingSourceHost
120Tests4°C Do Not FreezeBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Human WM-1 (Waldenström's macroglobulinemia) cell line.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human CD70 molecule at ~30kD. Inhibits T cell proliferation induced by dendritic cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hintzen, R.Q., et al., J. Immunol. 152: 1762-1773 (1994). 2. Bowman, M.R., et al., J. Immunol. 152: 1756-1761 (1994). 3. Leukocyte Typing V (S.F. Schlossman, et al., eds.) Oxford University Press, Oxford (1995) p.1137-1138. 4. Agematsu, K., et al., J. Immunol. 154: 3627-3635 (1995).