Technical Data
C2431-10
CD79a (B-cell)
Description:
A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines.

Applications:
Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 0.5-1ug/ml for 30 minutes at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Raji cells, lymph nodes and tonsils.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k0.N.163Affinity Purified
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide consisting of aa (GTYQDVGSLNIADVQ) from human CD79a protein. Cellular Localization: Cell membrane.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2431-10X.
Specificity:
Recognizes human CD79a at ~47kD. Species Crossreactivity: monkey, equine, bovine, porcine, rabbit, guinea pig, rat, mouse, chicken (Plasma cells). Does not react with opossum
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Astsaturov IA; Matutes E; Morilla R; Seon BK; Mason DY; Farahat N; Catovsky D. Leukemia, 1996 May, 10(5):769-73.2. 2. Ashton-Key M; Thorpe PA; Allen JP; Isaacson PG. American Journal of Surgical Pathology, 1995 Nov, 19(11):1294-9.