Technical Data
CD81 (TAPA-1, Target of anti-Proliferative Antibody-1)
CD81 has a very broad cellular distribution, being expressed on T and B lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells (1). Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive (1). CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82 (3). It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus (8). Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. For FunctionalStudies use C2433-10X. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Acetone fixed frozen sections
Optimal dilutions to be determined by the researcher.

Positive Control:
Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, human lymphocytes, tonsil and lymph node.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k0.N.165Affinity Purified
500ul-20CBlue IceHumanMouse
B cell line derived from a Burkitt lymphoma. Cellular Localization: Cell membrane
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2433-10X.
Recognizes human CD81/TAPA-1 at 24kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kishimoto, T., et al., eds. Leukocyte Typing VI, p187-189, Garland Publishing, New York (1997). 2. Takahashi, S., et al., J. Immunol. 145(7): 2207-2213 (1990). 3. Mannion, B.A., et al., J. Immunol. 157(5): 2039-2047 (1996). 4. Sato, S., et al., J. Immunol. 159(7): 3278-3287 (1997). 5. Horvath, G., et al., J. Biol. Chem. 273(46): 30,537-30,543 (1998). 6. Todd, S.C., et al., J. Exp. Med. 184(5): 2055-2060 (1996). 7. Levy, S., et al., Annu. Rev. Immunol. 16: 89-109 (1998). 8. Pileri, P., et al., Science 282: 938-941 (1998). 9. Schlossman, S.F., et al., eds. Leukocyte Typing V, Oxford University Press, Oxford (1995).