		Technical Data
C2438-05	CD86 (Activation B7-2 Antigen, B70, B7-2, BU63, CD28LG2, CTLA-4 Counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte Activation Antigen CD86 Precursor)

Description:
CD86 is also known as B7-2 and is the second ligand for CD28 and CTLA-4. It may have an important role in co-stimulation of T cells in the primary immune response.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1:20-1:100. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry (frozen): 1:20-1:100
Optimal dilutions to be determined by researcher.

Fusion Partners:
Spleen cells from immunized mice were fused with cells of the P3 X63 Ag8.653 myeloma cell line.

Positive Control Tissue (IHC):
Human Tonsil

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	3H1726	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human peripheral blood lymphocytes

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris buffered saline, pH 8.2, 0.09% sodium azide.

Specificity:

Recognizes CD86, a 70kD cell surface protein expressed by activated B cells and monocytes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Nozawa, Y. et al. (1993). J. Pathol. 169: 309.