Technical Data
C2438-05
CD86 (Activation B7-2 Antigen, B70, B7-2, BU63, CD28LG2, CTLA-4 Counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte Activation Antigen CD86 Precursor)
Description:
CD86 is also known as B7-2 and is the second ligand for CD28 and CTLA-4. It may have an important role in co-stimulation of T cells in the primary immune response.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1:20-1:100. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry (frozen): 1:20-1:100
Optimal dilutions to be determined by researcher.

Fusion Partners:
Spleen cells from immunized mice were fused with cells of the P3 X63 Ag8.653 myeloma cell line.

Positive Control Tissue (IHC):
Human Tonsil

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H1726Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human peripheral blood lymphocytes
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris buffered saline, pH 8.2, 0.09% sodium azide.
Specificity:
Recognizes CD86, a 70kD cell surface protein expressed by activated B cells and monocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nozawa, Y. et al. (1993). J. Pathol. 169: 309.