		Technical Data
C2438-20A	CD86 (Activation B7-2 Antigen, B70, B7-2, BU63, CD28LG2, CTLA-4 Counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte Activation Antigen CD86 Precursor)

Description:
CD86, also known as B70/B7-2, is a 80kD type I integral membrane protein and a member of the immunoglobulin superfamily. It is expressed at high levels on resting monocytes and dendritic cells and at very low levels on resting B and T lymphocytes. Activation of B cells, T cells and monocytes leads to upregulation of CD86 expression. Like CD80, CD86 is a ligand of CD28 and CD152. The interactions of CD86 (and CD80) with CD28 provides important costimulatory signals to T cells activated via the CD3/TCR complex. CD86 is expressed earlier than CD80 and appears to dominate in the primary immune response (1-3).

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1ug/10e6 cells
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	5K246	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.2mg/ml

Immunogen:

Human CD86 B7-2

Purity:

Purified

Form

Supplied as a liquid in 0.1M Tris buffered saline, pH 8.2 and a stabilizing agent.

Specificity:

Recognizes human CD86 B7-2

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford. 2. 210. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD86 Section, Academic Press, New York, p. 345 3. Nozawa, Y., E. Wachi, K. Tominaga, M. Abe, and H. Wakasa. 1993. J. Pathol. 169:309.