Technical Data
CD90 (Thy-1)
The affinity of the C2441-06L for rat Thy-1 is 3x10e9m-1 and for mouse Thy-1.1 is 3x10e8m-1. The Thy-1 antigen is found on a variety of cell types including thymocytes, neuronal cells, T-Lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue. The molecule is a glycoprotein with 112aa which is homologous to immunoglobulin domains. The binding characteristics have been extensively studied. Demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy and reported to induce glomerular nephritis in Wistar rats.

Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50-1:100. 10ul labels 10e6 cells in 100ul.
Immunohistochemistry: Frozen sections
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
Goat anti-mouse IgG labeled with FITC (rat absorbed):
I1904-03A: IgG, X-Adsorbed (FITC) Pab Dk xMo
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
Goat anti mouse IgG labeled with RPE (rat adsorbed)
I1903-12X: IgG, H&L, X-Adsorbed (PE) Pab Dk xMo

Recommended Negative Controls:
I1904-78Y: IgG1, Murine Negative Control (For rat tissue)

NS1 myeloma cells with spleen cells from Balb/c mice.

Source: Tissue culture supernatant

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG13H1751Affinity Purified
250ug-20CBlue IceRatMouse
Rat Thy 1 antigen
Purified by Protein G affinity chromatography
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Recognizes the rat Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus C2441-06L reacts with Thy-1.1 mice e.g. AKR and FBV strains, but not Thy-1.2 mice e.g. CBA, BALB/c. Species Crossreactivity: rabbit, mouse, and guinea pig.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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