Technical Data
C2441-85B
CD90.2 (Thy1.2, Thy 1b)
Description:
Thy-1 was discovered in early 1960s during the search for heterologous antisera against mouse leukemia cells. It was originally named theta ( ) antigen, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in thymocytes (precursors of T cells in the thymus). The human homolog was isolated in 1980 as a 25kDa protein (p25) of T-lymphoblastoid cell line MOLT-3 binding with anti-monkey-thymocyte antisera. The conserved gene and its alleles Thy-1 has been conserved throughout vertebrate evolution and even in some invertebrates, with homologs described in many species like squid, frogs, chickens, mice, rats, dogs, and humans. The Thy-1 gene is located at human chromosome 11q22.3 (mouse chromosome 9qA5.1). Some believe that there may be a functional significance of both this gene and CD3 delta subunit (T3D) mapping to chromosome 11q in man and chromosome 9 in mouse, though there is no homology (in fact this speculation lead to its localization in chromosome 11q-the human chromosome region syntenic to mouse chromosome 9 which harbored T3D). In mouse there are two alleles Thy1.1 (Thy 1a, CD90.1) and Thy1.2 (Thy 1b, CD90.2) which differ by one amino acid at position 108 (arginine in Thy-1.1 and glutamine in Thy-1.2). Thy 1.2 is expressed by most strains of mice, whereas Thy1.1 is expressed by some like AKR/J and PL mouse strains.

F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed)

Fusion Partners: Spleen cells from immunized Sprague-Dawley rats were fused with cells from the mouse SP2/0-Ag14 myeloma cell line.

Recommended Negative Controls: Rat IgG1 Negative Control

Applications:
Flow Cytometry: 1:50-1:100
Immunohistochemistry (frozen)
Immunoprecipitation
Western Blot
Optimal working dilutions to be determined by researcher.
TypeIsotypeCloneGrade
MabIgG13H3036Affinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
EL-4 cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the mouse Thy1.2 cell surface antigen and binds to Tcell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody. This clone recognizes a 30kD glycoprotein in immunoprecipitation and Western blotting assays. The antibody strongly induces homotypic adhesion of Thy1.2 positive cell lines.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fan, X. et al (1996). ICAM-1, -2, and LFA-1 independent homotypic T cell aggregation induced by a novel activating monoclonal antibody targeting the murine Thy-1 molecule. Inflammation 20: 401.