		Technical Data
C2441-97A	CD91, 85kD (LRP, alpha2MR, Low Density Lipoprotein Receptor-related Protein)

Description:
A strong immunohistochemical reaction is seen in hepatocytes, tissue macrophages, subsets of neurones and astrocytes in the central nervous system, fibroblasts, smooth muscle cells, and monocyte-derived foam cells in atherosclerotic lesions in the arterial wall. Can also be used for the characterization of a subset of myelomonocytic subtypes of chronic and acute leukemia (CD 91).

Applications:
Suitable for use in Immunofluorescence, Immunohistochemistry, Flow Cytometry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:20
Optimal dilutions to be determined by researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	5F140	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.05mg/ml (after reconstitution)

Immunogen:

Human CD91

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA. Reconstitute with 1ml sterile ddH2O.

Specificity:

Recognizes the 85kD b-chain of human CD91 expressed in a restricted spectrum of cell types. Species Crossreactivity: rabbit, rat, hamster and mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Strickland et al. (1990) J Biol Chem 265:17401-17404. 2. Coukos et al (1994): Am J Pathology 144:383-392. 3. Rebeck et al (1993): Neuron 11:575-580. 4. Gonias et al (1992): Am J Pathology 141:37-42. 5. Strickland DK, Kounnas MZ, Argraves WS (1995) FASEB J 9:890-898 6. Herz J and Strickland DK (2001) J. Clin. Invest. 108:779-784.