Technical Data
C2444-01
CD99 (MIC2, p30/32MIC2, Ewing’s Sarcoma Marker, E2 Antigen)
Description:
CD99 antigen is expressed on most hematological cells, especially at high density on thymocytes, T cells and the cells of T-cell leukemias and lymphomas and is differentially expressed on early hematopoietic stages, but lacking on fetal B cells and some B cell lines, eosinophils, granulocytes and NK-cell line YT. CD99 antigen may be involved in T-cell adhesion processes and may have a role in hematopoietic adhesion pathways.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, and Immunohistochemistry. Other applications have not been tested.

Recomended Dilution:
Immunofluorescence: 0.125ug/10e6 cells or 100ul peripheral blood.
Flow Cytometry: 0.125ug/10e6 cells or 100ul peripheral blood
Immunohistochemistry: Acetone-fixed frozen and formalin-fixed paraffin.
Immunohistochemistry: frozen (acetone-fixed) and paraffin sections.
Optimal dilutions to be determined by researcher.


Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a5F56Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
As reported
Immunogen:
Human CD99
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.0, 0.1% sodium azide before the addition of glycerol to 40%.
Specificity:
Recognizes a 32kD type I single chain transmembrane glycoprotein, called E2 antigen which is the MIC2 gene product. Reactivity: Normal human peripheral blood lymphocytes: 99%; Monocytes: 95%. Does not react with normal human peripheral blood granulocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tadamitsu K, K. Hitoshi, A.E.G.Kr. van dem Borne, et al., eds. 1997. Leukocyte Typing VI: White Cell Differentiation Antigens. P 751183 Garland Publishing, Inc., New York.