Technical Data
C2445-30B
CD101 (PE)
Description:
CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat -1:5. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: frozen samples
Optimal dilutions to be determined by the researcher.

Recommended Negative Controls:
I1904-79D: IgG1 Murine Negative Control (PE)

Storage and Stability:
Lyophilized powder may be stored at 4C. Reconstitute with ddH2O. Aliquot and store at 4C. Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG14j29Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human thymic clone B12
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml ddH2O.
Specificity:
Recognizes human CD101. Reported to inhibit allogeneic T-cell responses (1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bagot, M. et al . (1997). CD101 is expressed by skin dendritic cells. Tissue Antigens. 50: 439 - 448.