Technical Data
C2445-63J
CD103 (PE/CY5)
Description:
CD103 is the integrin alphaE subunit which usually forms a heterodimer with the integrin beta7 subunit. It is expressed on intraepithelial lymphocytes and 1-2% of peripheral blood lymphocytes. CD103 binds E-cadherin on epithelial cells. This interaction may be of importance in the homing and retention of CD103+ lymphocytes in the intestinal epithelium (1-4). PE/Cy5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 5ul/10e6 cells
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
TypeIsotypeCloneGrade
MabIgG15K293Purified
SizeStorageShippingSourceHost
100 Tests4°C Do not freezeBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with PE/CY5.
Specificity:
Recognizes human CD103.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kishimoto, T., A.E.G. von dern Borne, S.M. Goyert, D.Y. Mason, M. Miyasaka, L. Moretta, K. Okumura, S. Shaw, T.A. Springer, K. Sugamura, and H. Zola, eds. 1998. Leukocyte Typing VI: White Cell Differentiation Antigens, Academic Press, New York. 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD103 Section, Academic Press, New York, p. 379. 3. Shaw, S.K., et al., J. Biol. Chem. 269: 6016 (1994). 4. Shaw, S.K., & Brenner, M.B., Semin. Immunol. 7: 335 (1995).