Technical Data
C2445-63R
CD103 (Integrin alpha E, Integrin alpha M290, Itgae, Integrin alpha E, Epithelial-associated, A530055J10, alpha-E1, alpha-M290, aM290, MGC183601)
Description:
CD103 is a type I transmembrane glycoprotein known as aE integrin or Integrin aIEL chain. It belongs to integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-b stimulation. In association with integrin b7, CD103 is expressed as aE/b7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150kD (C-terminal) chain and a 25kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2E7Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseHamster
Concentration:
~0.5mg/ml
Immunogen:
Mouse intestinal intraepithelial lymphocytes
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes mouse CD103.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201. 2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852. 3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635. 4. Sung SS, et al. 2006. J. Immunol. 176:2161. 5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063. 6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.