Technical Data
CD104 (Integrin beta4)
Suitable for use in Flow Cytometry, Immunoprecipitation, Inhibition of cell attachment. Other applications not tested.

Recommended Dilution:
Inhibition of cell attachment: inhibits SCC9 cell adhesion to human and EHS laminin, but not to laminin GD-2 peptide, type IV collagen or fibronectin.
Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.
Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
Immunoprecipitation: precipitates PAGE bands of 66, 76, 120, 135 and 200kD (reduced) or 66, 110, 125, 130 and 190kD (non-reduced) from surface-biotinylated SCC9 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a,k2Q792Affinity Purified
100ug-20CBlue IceHumanMouse
Human squamous cell carcinoma cells (SCC9)
Purified immunoglobulin from Protein A Sepharose chromatography
Supplied as a liquid in 0.02M PB pH 7.6, 0.25M sodium chloride containing 0.09% sodium azide
Human b4 integrin (CD104)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pattaramalai, S., et al. Exper. Cell Res. 222: 281290 (1996). 2. Tissue Antigens 48:356 (1996).