Technical Data
CD104 (Integrin beta4)
Human beta4 integrin, also known as CD104, is a 205kD glycoprotein. It associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines.

Suitable for use in Inhibition of Cell Attachment, Immunohistochemistry, Flow Cytometry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Inhibition of cell attachment: Inhibits SCC9 cell adhesion to human laminin and laminin GD-2 peptide, but not to EHS laminin, type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.
Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
Immunoprecipitation: Precipitates PAGE bands of 66, 76, 120, 135 and 200kD (reduced) or 66, 110, 125, 130 and 190kD (non-reduced) from surface-biotinylated SCC9 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k2Q794Affinity Purified
100ug-20CBlue IceHumanMouse
Human squamous cell carcinoma cells (SCC9)
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.6.
Recognizes human b4 integrin (CD104)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pattaramalai, S., et al. Exper. Cell Res. 222: 281290 (1996). 2. Tissue Antigens 48:356 (1996).