Technical Data
C2446-01A
CD104 (Integrin beta4)
Description:
Human beta4 integrin, also known as CD104, is a 205kD glycoprotein. It associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines.

Applications:
Suitable for use in Inhibition of Cell Attachment, Immunohistochemistry, Flow Cytometry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Inhibition of cell attachment: Inhibits SCC9 cell adhesion to human laminin and laminin GD-2 peptide, but not to EHS laminin, type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.
Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
Immunoprecipitation: Precipitates PAGE bands of 66, 76, 120, 135 and 200kD (reduced) or 66, 110, 125, 130 and 190kD (non-reduced) from surface-biotinylated SCC9 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2Q794Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human squamous cell carcinoma cells (SCC9)
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6.
Specificity:
Recognizes human b4 integrin (CD104)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pattaramalai, S., et al. Exper. Cell Res. 222: 281290 (1996). 2. Tissue Antigens 48:356 (1996).