Technical Data
CD104 (Integrin beta4)
Suitable for use in Immunohistochemistry, Flow Cytometry, Inhibition of Cell Attchment and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.
Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
Inhibition of Cell Attachment: Inhibits SCC9 cell adhesion to human and EHS laminin and laminin GD-2 peptide, but not to type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
Immunoprecipitation: Precipitates PAGE bands of 66, 76, 120, 135 and 200kD (reduced) or 66, 110, 125, 130 and 190kD (non-reduced) from surface-biotinylated SCC9 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG1,k2Q793Affinity Purified
100ug4C (-20C Glycerol)Blue IceHumanMouse
Human squamous cell carcinoma cells (SCC9)
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS pH 7.6, 0.09% sodium azide
Recognizes human b4 integrin (CD104)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pattaramalai, S., et al. Exper. Cell Res. 222: 281290 (1996). 2. Tissue Antigens 48:356 (1996).