Technical Data
C2446-01J
CD104 (Integrin beta4)
Description:
Integrins are alpha/beta heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 alpha and 8 beta subunits that form 24 known integrins with distinct tissue distribution and over- lapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside- out signaling) (1,2). Integrin beta 4 pairs with integrin 6 on the cell surface mem- brane to form the integrin alpha6beta4 heterodimer, an important laminin receptor that is essential for hemidesmosome formation and the support of stable adhesions between basal epithelial cells and the basement membrane (4,5). Integrin beta 4 is an important component in several growth factor induced signaling pathways that are involved in tumorigenesis and invasive cell growth (6,7).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Immunogen:
Synthetic peptide (KLH- coupled) corresponding to residues surrounding Ser1812 of human integrin 4 protein.
Purity:
Purified peptide affinity chromatography.
Form
Supplied as a liquid in 10mM HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
Specificity:
Detects endogenous levels of total human integrin beta 4 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liu, S. et al. (2000) J Cell Sci 113(20):356371. 2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2:91100. 3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305:28598. 4. Schaapveld, R.Q. et al. (1998) J Cell Biol 142:27184. 5. Litjens, S.H. et al. (2006) Trends Cell Biol 16:37683. 6. Guo, W. et al. (2006) Cell 126:489502. 7. Bertotti, A. et al. (2006) J Cell Biol 175:9931003.