Technical Data
C2446-50B
CD105 (Endoglin, TGF beta1,3 Receptor, gp160)
Description:
Endoglin is an endothelial homodimeric membrane antigen containing the tripeptide sequence RGD which is a recognition motif for adhesion receptors of the integrin family. CD105 is a Type I transmembrane protein and exists as an O-and N-glycosylated homodimer. Endoglin is a TGF beta-binding glycoprotein that is most abundantly expressed on endothelial cells in tissues with in ammatory or in ammatory-like characteristics, such as the skin lesions in psoriasis. Proliferating endothelial cells in granulation tissue and in tumors (e.g. melanoma of the skin) also exhibit enhanced expression of endoglin. In addition, a marked staining of syncytiotrophoblasts in placenta is observed. CD105 binds to TGF-b1 and b3 with high affinity but not TGF-b2.

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 1:100 for 60 min at RT. For staining of formalin-fixed tissues, digest sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37C.
Optimal dilution determined by the researcher.

Positive Control:
HUVEC cells. Tonsil, lymph node and thymus.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3F106Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Purified gp160 from cell membrane glycoproteins of fresh non-T/ non-B acute lymphoblastic leukemia cells. MW of Antigen: 95kD (monomer) and 190kD (dimer). Cellular Localization: Cell membrane.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide at 1mg/ml. See C2446-50BX.
Specificity:
Recognizes human CD105 (Endoglin, TGF beta1/3 Receptor).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.