Technical Data
C2446-50E
CD105 (Endoglin, gp160, TGF beta1,3 Receptor)
Description:
Human CD105 (endoglin) is a homodimeric membrane glycoprotein virtually restricted to endothelial cells, and present on some leukemia cells and minor subsets of bone marrow cells. CD105 binds to TGF-b1 and b3 with high affinity.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow cytometry: 10ug/ml
Immunohistochemistry: frozen sections
Optimal dilutions to be determined by the researcher.

Flow cyytometry Protocol:
1. Five x 10e5 cultured Nalm-6 human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2446-50E at a concentration of 10ug/ml.
2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC
3. After which they were washed three times, fixed and analyzed by FACS.
4. Cells stained positive with a mean shift of 0.79 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k7H217Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Membrane preparation of human B-lineage leukemia cells
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml Gentamicin Sulfate.
Specificity:
Recognizes human CD105 homodimer with a molecular weight of ~160kD. Antibody will augment binding of TGF-b1 to CD105 expressing leukemia cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Y. Haruta & B.K. Seon, (1986) Proc Natl Acad Sci USA 83: 7898-7902. 2. H. Hara, et al, (1988)Cancer Res 48: 4673-4680. 3. W.C. Biddle, et al, (1989) Leukemia Res 13: 699-707. 4. S. Cheifetz, et al, (1992) J Biol Chem 267: 19027-19030. 5. B.K. Seon, et al, (1995) Proc Am Assoc Can Res 36: 23. 6. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1756-1761, 1762-1764. 7. B.K. Seon, et al (1996) Tissue Antigens 48: 417.