Technical Data
C2446-50E2
CD105 (Endoglin, gp160, TGF beta1,3 Receptor, HHT1, ORW1, Osler Rendu Weber syndrome 1) (FITC)
Description:
CD105 is a disulfide-linked homodimeric type I transmembrane glycoprotein (1, 2). Two isoforms of CD105, a short form and a long form, differ by 33aa in their cytoplasmic tails (3). The long form (180kD in size) is considered the predominant form of the protein although both isoforms have been detected by RT-PCR in endothelial cells, monocytic cell lines, and placenta (3, 4). CD105 serves as the regulatory component of the TGF-beta receptor complex by associating with TGF-b receptor I and II (5, 6). These receptor complexes efficiently bind TGF-b1 and TGF-b3 with high affinity (5, 7). CD105 also binds other members of the TGF-b superfamily including activin-A, BMP-7 and BMP-2 (5). Functionally, CD105 plays an important role in angiogenesis (7, 8), hematopoiesis (9, 10) and cardiac development (9). CD105 was also shown to be a functional marker that defines long-term repopulating hematopoietic stem cells from the bone marrow "side-population" cells (11). Multipotent stem cells have been isolated from bone marrow and muscle tissue based on their expression of CD105 (12, 13). Clinically, mutations in the gene for CD105 result in the autosomal dominant disorder Hereditary Hemorrhagic Telangiectasia Type 1 (14). In addition, soluble levels of shed CD105 have been associated with poor prognosis in breast, colorectal and other types of cancer (15, 16). Further investigations may elucidate the role of CD105 and its ligands in a variety of biologic processes.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Will augment binding of TGF-b1 to CD105 expressing leukemia cells. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k7H217Affinity Purified
SizeStorageShippingSourceHost
120Tests-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Membrane preparation of human B-lineage leukemia cells
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:
Recognizes human CD105 homodimer at ~160kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Letarte, M. et al. (1995) Leukocyte Typing V, Schlossman, S.F. et al. Eds., Oxford University Press, Oxford. 2. Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361. 3. Bellon, T. et al. (1993) Eur. J. Immunol. 23:2340. 4. Li, C. et al. (2000) FASEB J. 14:55. 5. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584. 6. Guerro-Esteo, M. et al. (2002) J. Biol. Chem. 277:29197. 7. Cheifetz, S. et al. (1992) J. Biol. Chem. 267:19027. 8. Duff, S.E. et al. (2003) FASEB J. 17:984. 9. Arthur, H.M. et al. (2000) Dev. Biol. 217:42. 10. Pierelli, L. et al. (2001) Leuk. Lymphoma 42:1195. 11. Chen, C.Z. et al. (2002) Proc. Natl. Acad. Sci. USA 99:15468. 12. Lodie, T.A. et al. (2002) Tissue Eng. 8:739. 13. Dell'Agnola, C. et al. (2002) Exp. Hematol. 30:905. 14. McAlister, K.A. et al. (1994) Nat. Genet. 8:345. 15. Takahashi, N. et al. (2001) Clin. Cancer Res. 7:524. 16. Calabro, L. et al. (2003) J. Cell Physiol. 194:171.