Technical Data
C2446-71E
CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (PE)
Description:
CD106, also known as INCAM-1, is a 100-110kD vascular adhesion cell adhesion molecule-1 (VCAM-1) that is member of the immunoglobulin superfamily. CD106 is expressed predominantly on cytokine-activated vascular endothelium but has also been identified on interfollicular dendritic cells, some macrophages and bone marrow stromal cells. Endothelial CD106 binds the integrins alpha4beta1 (CD49d/CD29, VLA-4) and alpha4beta7 and contributes to extravasation of lymphocytes, monocytes, basophils and eosinophils (but not neutrophils) from blood vessels, particularly at sites of inflammation. Unlike the beta2 integrins, the CD106-VLA-4 interaction can mediate both the initial tethering and rolling of lymphocytes on endothelium as well as their subsequent arrest and firm adhesion. CD106 expressed on non-vascular tissues has been implicated in the interaction of hematopoietic progenitors with bone marrow stromal cells, B cell binding to follicular dendritic cells, costimulation of T cells, and embryonic development. This antibody inhibits in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul/10e6 cells
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Stable for 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG15K267Purified
SizeStorageShippingSourceHost
100Tests4C Do not freezeBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human CD106 (VCAM-1).
Purity:
Purified
Form
Supplied as a liquid in PBS, sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human CD106/VCAM-1 at 100-110kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Xiao, J. et al., (2012) Am J Cardiovasc Dis 2; 1-11. 1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford. 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD106 Section, Academic Press, New York, p. 386. 3. Thornhill, M.H., S.M. Wellicome, D.L. Mahiouz, J.S. Lanchbury, U. Kyan-Aung, and D.O. Haskard. 1991. J. Immunol. 146:592. 4. Bevilacqua, M.P. 1993. Annu. Rev. Immunol. 11:767.