Technical Data
C2447-52B
CD115 (c-fms, Fms, CSF-1R, M-CSFR)
Description:
CD115, also known as c-fms, macrophage colony stimulating factor receptor (M-CSFR), or CSF-1R, exists in immature (gp130c-fms) and mature (gp150c-fms) forms. CSF-1R exhibits an intrinsic tyrosine-specific protein kinase activity. It is expressed on the cells of the mononuclear phagocyte lineage. CSF-1R is an integral transmembrane glycoprotein consisting of a 512 amino acid extracellular domain that includes the CSF-1 binding site, a single 25 amino acid membrane-spanning segment, and a 435 amino acid cytoplasmic portion that includes the tyrosine kinase domain.

Applications:
Suitable for use in Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunocytochemistry: Fresh, frozen or formalin-fixed paraffin- embedded tissue sections.
Immunohistochemistry: Formalin-fixed paraffin-embedded tissue sections. Antigen retrieval with heating in citrate buffer recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceFelineRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide: Ser-Leu-Pro-Arg-Leu-Lys-Arg-Ser-Glu-Ser-Gly- Arg-Tyr selected from the external domain of the feline v-fms sequence (amino acids 399-411).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10mM sodium azide.
Specificity:
Recognizes human c-fms oncoprotein. Species crossreactivity: mouse macrophages.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.