Technical Data
C2448-02A
CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)
Description:
c-Kit is a transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological processes including cell proliferation, apoptosis, chemotaxis and adhesion. It plays a critical role in activation and growth in a number of cell types such as hematopoietic stem cells, mast cells, melanocytes and germ cells. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.

Applications:
Suitable for use in Immunohistochemistry, Flow Cytometry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:100-1:400
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa963-976, TASSSQPLLVHDDV, of human CD117 (c-kit).
Purity:
Purified by affinity chromatography
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human CD117 (c-kit). Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chan, K.K., et al., Arterioscler. Thromb. Vasc. Biol. 29: 1060-1066 (2009). 2. Inngjerdingen, M., et al., The Journal of Immunology 188(6): 2499-2508 (2012). 3. Harhouri, K., et al., Blood 115: 3843 - 3851 (2010).