Technical Data
CD117, Recombinant, Human (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)
Molecular Biology Storage: -70CShipping: Dry Ice
C-Kit is the receptor tyrosine kinase for stem cell factor (SCF) and is a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors (1,2). It plays critical controlling roles in a number of cell types such as hematopoietic stem cells, mast cells, melanocytes and germ cells (3). Upon binding with its ligand, c-Kit receptors are induced undergoing dimerization/ oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCgamma, p85 subunit of PI3 kinase, SHP2 and CrkL (4), which links c-Kit directly to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), while mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyrosine 719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).

Human Kit (GenBank Accession No. BC071593), (a.a. 544-end) with N-terminal His tag,

MW 53.4kD, expressed in an Baculovirus infected Sf9 cell expression system.

Specific Activity: 15 U/mg. One unit is defined as the amount of enzyme that will incorporate 1nmol phosphate to the tyrosine substrate per minute at pH 7.4 and 30C.

Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1mM ATP, 30ug/ml Poly (Glu:Tyr)4:1 substrate, and 4ug /ml recombinant c-Kit.

Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Stability: >6 months at 80C

Molecular Weight:
Source: Recombinant, human from Baculovirus infected Sf9 cells
Purity: 90% by SDS-PAGE.
Form: 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Yarden,Y., etal., EMBO J. 6 (11), 3341-3351 (1987).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.