Technical Data
CD126 (Interleukin 6 Receptor, neutralizing, IL-6R)
The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component speci c to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low af nity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity. CD126 is single chain type I transmembrane protein with Mr=130140kD and is a common receptor subunit for many cytokines, forming complexes with CD126, IL-11R, LIF-R, and other proteins. These gp130-containing complexes mediate signaling of oncostatin M, IL-6, IL-11, LIF, CNF, and cardiolipotrophin 1. gp130 is expressed at low levels by most cell types. This antibody was recognized as reacting with gp130 at the Sixth Leucocyte Typing Workshop.

Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen tissues
Flow Cytometry: 1:50; Use 10ul per 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG12Q1481Highly Purified
100ug-20CBlue IceHumanMouse
CHO cells transfected with cDNA of the human IL-6 receptor.
Purified by Ion Exchange chromatography
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Recognizes both the soluble and membrane bound human IL-6 receptor. Inhibits IL-6 mediated proliferation of XG-1 cells and partially blocks the binding of IL-6 to its receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Clement C et al. in CR.3.2 Leucocyte Typing, Boston (1993).