Technical Data
C2464
CD126 (Interleukin 6 Receptor, neutralizing, IL-6R)
Description:
The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component speci c to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low af nity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity. CD126 is single chain type I transmembrane protein with Mr=130140kD and is a common receptor subunit for many cytokines, forming complexes with CD126, IL-11R, LIF-R, and other proteins. These gp130-containing complexes mediate signaling of oncostatin M, IL-6, IL-11, LIF, CNF, and cardiolipotrophin 1. gp130 is expressed at low levels by most cell types. This antibody was recognized as reacting with gp130 at the Sixth Leucocyte Typing Workshop.

Applications:
Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen tissues
Flow Cytometry: 1:50; Use 10ul per 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q1481Highly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
CHO cells transfected with cDNA of the human IL-6 receptor.
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes both the soluble and membrane bound human IL-6 receptor. B-R6 will inhibit IL-6 mediated proliferation of XG-1 cells and partially blocks the binding of IL-6 to its receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Clement C et al. in CR.3.2 Leucocyte Typing, Boston (1993).