Technical Data
C2464-01
CD126 (Interleukin 6 Receptor, IL-6R)
Description:
The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component speci c to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low af nity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA, Immunoprecipitation and Inhibition assays. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50 Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cellls from immunized BALB/c (Iffa Credo) mice were fused with the X63/Ag.8653 mouse myeloma cell line.

Recommended Secondary Reagents:
I1904-66A IgG, F(ab’)2, X-Adsorbed (FITC)

Recommended Negative Controls:
I1904-78R: IgG1 Murine Negative Control

Storage and Stability::
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H1836Highly Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
CHO cells transfected with cDNA of the IL-6 receptor.
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Removal of sodium azide is recommended prior to use in functional assays.
Specificity:
Recognizes soluble and membranous human IL-6R. This antibody has been shown to inhibit IL-6 mediated proliferation of XG-1 cells. It partially blocks binding of IL-6 to its receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Durandy, A. et al. (1994) Role of IL-6 in promoting growth of human EBV-induced B-cell tumors in severe combined immunodeficient mice. J. Immunol. 152:5361-53672. Lu, Z. et al. (1995) Interleukin-10 is a proliferation factor but not a differentiation factor for human myeloma cells. Blood 85:2521-25273. Keller, P. et al. (2005) Interleukin-6 receptor expression in contracting human skeletal muscle: regulating role of IL-6. FASEB J. 19:1181-1183.