Technical Data
C2500-15
CEA Receptor, Long (CEARL, Nuclear Ribonucleoprotein, nRNP, hnRNP M4 isoform 1, Heterogeneous Nuclear Ribonucleoprotein A0)
Description:
Applications:
Suitable for use in Western Blot, Immunocytochemistry and FACS Analysis. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.5ug/ml Detects CEARL in RIPA lysates from HeLa cells.
Immunocytochemistry: 1:1000 Reported to show positive immunostaining for CEARL in rat Kupffer cells.
FACS Analysis: Reported to detect CEARL in rat Kupffer cells by FACS analysis.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa161-182 [MATTGGMGMGPGGPGMITIPPS] of human carcinoembryonic antigen receptor “long” (CEARL), conjugated to KLH.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity:
Recognizes human CEARL/hnRNP M4 isoform 1, Mr ~74-80kD. Additional proteins were also detected, Mr ~55 and 70kD. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Weichselbaumer, M. et al., (2011) PLoS Curr. doi: 10.1371/currents.RRN1223. 1. Bajenova, O., et al., Exp. Cell Res. (submitted 4/03).