Technical Data
C2514-90H
CD133 (Biotin) (AC133, Antigen AC133, CD133, CD133 Antigen, CD133 Retinal Degeneration, CORD12, MCDR2, MSTP061, Prominin 1, Prominin-1, PROM1, PROM, Prominin-like Protein 1, PROML1, RP41, STGD4)
Description:
CD133 (also known as Prominin-1 or AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not conclusive, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Applications:
Suitable for use in Flow Cytometry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1.0ug / 10e6 cells in 100ul volume
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,l12J16Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Specificity:
Recognizes mouse CD133.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Singh SK, et al. 2004. Nature 18:396. 2. Falini B, et al. 2005. New Engl. J. Med. 352:254. 3. Bonanno G, et al. 2004. Transfusion 44:1087. 4. Gaipa G, et al. 2002. Haematologica 87:347. 5. Wuchter C, et al. 2001. Haematologica 86:154. 6. Zhang Y, et al. 2012. Diabetes. 61:2114.