Technical Data
C2548-78A
CD146 (Mucin 18, MUC18 Glycoprotein, Melanoma Associated Antigen A32, S-endo 1 Endothelial Associated Antigen, Melanoma Adhesion Molecule Mel-CM) (Biotin)
Description:
This antibody does not stain carcinoma cell lines HT-29, COLO205 or M21; the T cell lines Jurkat and HuT78; fibroblasts; HL-60 or CHO cells; or EBV transformed B cell lines, although it is expected that CD146 expression is present in many tumor lines. It does not stain normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain are positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line
(HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation. This biotinylated format is particularly useful for studies on mouse tissues when combined with streptavidin-FITC.

Applications:
Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1-10ug/ml
ELISA: 1-10ug/ml
Immunoprecipitation: 1-10ug/ml
Immunohistochemistry (Light paraformaldehyde fixation): 1-10ug/ml
Immunocytochemistry:1-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q401Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
HUVEC
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA, 0.1% sodium azide. Labeled with Biotin.
Specificity:
Recognizes human CD146. Reacts with endothelial cells in blood and marrow. Species Crossreactivity: canine, rabbit and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Sloboda, N. et al., (2012) J Gerontol A Biol Sci Med Sci, doi: 10.1093/gerona/glr236. 1. Solovey, A., et al., New England Journal of Medicine 337: 1584 (1997). 2. Gui, et al., Blood, Supplement Abstract #697 (1992). 3. St. Croix, et al., Science 289: 1197-1202 (2000).