Technical Data
C2548-81
CD147 (Extracellular Matrix Metalloproteinase Inducer, Basigin, Neurothelin, 5A11 Antigen, 5F7, Blood-brain Barrier HT7 Antigen, Collagenase Stimulatory Factor, Emmprin, Leukocyte Activation Antigen M6, M6, OK, OK Blood Group Antigen, TCSF, Tumor Cell Der
Description:
The antigen is also present on some endothelia [2]. CD147 is a membrane glycoprotein of MW 42-48kD expressed at high levels on activated lymphocytes but lower levels on resting cells. The antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue [2].It is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry (Neat): 1:20. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry (frozen): 1:20
Optimal working dilutions to be determined by researcher.

Hybridoma:
NSO/1 myeloma cells with spleen cells from Balb/c mice.

Recommended Negative Control:
I1904-78R: IgG1 Negative Control

Recommended Secondary Antibody:
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H1875Supernatant
SizeStorageShippingSourceHost
2ml-20°CBlue IceRatMouse
Concentration:
~10mg/ml
Immunogen:
T blasts from a mixed lymphocyte reaction.
Purity:
Tissue Culture Supernatant.
Form
Supplied as a liquid in 0.2M Tris HCl pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
Specificity:
Recognizes rat CD147.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Paterson, D.J. et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol. Immunol. 24:1281-1290. 2. Fossum, S. et al. (1991) The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence. Eur. J. Immunol. 21:671-679. 3. Cassella, J.P. et al. (1996) Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels. J. Anat. 189:407-415. 4. Foda, H. et al. (2001) Ventilator-induced lung injury upregulates and activates gelatinases and EMMPRIN: attenuation by the synthetic matrix metalloproteinase inhibitor, Prinomastat (AG3340). Am. J. Respir. Cell. Mol. Biol. 25 717-724. 5. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. stem Cells Dev. 18:1389-98.