Technical Data
C2548-83K
CD152 (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4)
Description:
CD152 is a 45kD glycoprotein, expressed normally as a disulphide-linked homodimer. It is expressed transiently on the surface of T cells following activation (peaking at 3 days), but is often present with the cytoplasm. CD152 is a ligand for CD80 and CD86, and is important in cellular interactions during the immune response.

Applications:
Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:10,.Use 10ul to label 10e6 cells in 100ul. Membrane permeabilisation is required.
Immunohistochemistry (Frozen): 5-50ug/ml Not suitable for use on paraffin.
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
F(ab')2 rabbit anti-mouse IgG HRP
F(ab')2 rabbit anti mouse IgG: RPE conjugate

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3/X63-Ag8.653 myeloma cell line.

Recommended Negative Controls:
I1904-83P1: IgG2a Mouse Negative Control

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a3H1883Affinity Purified
SizeStorageShippingSourceHost
200ug 4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human CTLA-4/human IgG heavy chain fusion protein.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 0.5% BSA.
Specificity:
Recognizes the human CD152 cell surface antigen, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Linsley, P.S., et al., (1992) J. Exp. Med. 176: 1,595-1,604. 2. Kuiper, H.M., et al., (1995) J. Immunol. 155: 1,776-1,783. 3. Castan, J., et al., (1997) Immunology 90: 265-271.