Technical Data
CD152 (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4)
CD152 is a 45kD glycoprotein, expressed normally as a disulphide-linked homodimer. It is expressed transiently on the surface of T cells following activation (peaking at 3 days), but is often present with the cytoplasm. CD152 is a ligand for CD80 and CD86, and is important in cellular interactions during the immune response.

Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:10. Use 10ul to label 10e6 cells in 100ul. Membrane permeabilisation is required.
Immunohistochemistry (Frozen): 5-50ug/ml Not suitable for use on paraffin.
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
F(ab')2 rabbit anti-mouse IgG HRP
F(ab')2 rabbit anti mouse IgG: RPE conjugate

Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3/X63-Ag8.653 myeloma cell line.

Recommended Negative Controls:
I1904-83P1: IgG2a Mouse Negative Control

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2a3H1883Affinity Purified
200ug 4C (-20C Glycerol)Blue IceHumanMouse
Human CTLA-4/human IgG heavy chain fusion protein.
Purified by affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide, 0.5% BSA.
Recognizes the human CD152 cell surface antigen, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Linsley, P.S., et al., (1992) J. Exp. Med. 176: 1,595-1,604. 2. Kuiper, H.M., et al., (1995) J. Immunol. 155: 1,776-1,783. 3. Castan, J., et al., (1997) Immunology 90: 265-271.