Technical Data
C2548-83K
CD152 (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4)
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Technical Data |
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C2548-83K |
CD152 (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) |
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Description: CD152 is a 45kD glycoprotein, expressed normally as a disulphide-linked homodimer. It is expressed transiently on the surface of T cells following activation (peaking at 3 days), but is often present with the cytoplasm. CD152 is a ligand for CD80 and CD86, and is important in cellular interactions during the immune response. Applications: Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10,.Use 10ul to label 10e6 cells in 100ul. Membrane permeabilisation is required. Immunohistochemistry (Frozen): 5-50ug/ml Not suitable for use on paraffin. Optimal dilution determined by the researcher. Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP F(ab')2 rabbit anti mouse IgG: RPE conjugate Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3/X63-Ag8.653 myeloma cell line. Recommended Negative Controls: I1904-83P1: IgG2a Mouse Negative Control Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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