Technical Data
C2548-98H
CD235ab (Glycophorin A/B, GpA, GpB)
Description:
CD235a (Glycophorin A (GpA)) antigen is exclusively expressed on human erythroid cells and their progenitors. These antibodies may be effectively used for differentiation in acute leukemias e.g. presence of the CD235a antigen on leukemia cells indicates an early erythroid cell lineage of the tumor cells. CD235a antibodies are used to identify erythroid cells during hematopoietic differentiation. CD235a is not expressed on lymphoid or granulocytic progenitor cells and therefore is very useful as a marker for detection of the erythroid cell lineage. CD235a seems to be absent from erythroid colony forming cells in bone marrow, but is present during maturation from pro-erythroid blast cells to mature erythrocytes. CD235a is clinically important in the classification of leukemias. Using Anti-CD235a antibodies, a distinction can be made between erythroleukemia and other (acute) leukemias like e.g. myeloid, lymphoid or undifferentiated leukemias. Anti-CD235a is often used in combination with detection of H-antigen and/or anti-CD36 antibodies for additional characterization of early erythroid cells. Additional immunophenotyping of early erythroleukemias can be performed by detecting myeloid-associated antigens such as CD13, CD14, CD15, CD33 and CD34, to discriminate from lymphoid lineage-associated antigens like CD2, CD7, CD10 and CD19.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: Indirect
Flow Cytometry: 0.015ug/ 100ul peripheral blood
Immunohistochemistry: Staining of acetone-fixed frozen sections and formalin–fixed paraffin sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k5F60Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human CD235ab (Glycophorin A/B, GpA, GpB)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoprotein. Reacts with normal human RBC (95.9%). Does not react with peripheral blood lymphocytes, granulocytes and monocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shen DC., Tang MH., Zhang JX., et al., 1993. HIR2: A monoclonal antibody against glycophorin A. J. of Monoclonal Antibody. 1993: 9(2): 59. 2. David M., A. Pascale, B. Armand, et al., 2002. Leukocyte Typing VII: White Cell Differentiation Antigens. P577-582.
General references: 1. Andersson, L.C. et al. 1979. Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia. Int.J.Cancer, 23, 717-720 2. Loken, M.R., et al., 1987. Flow cytometric analysis of human bone marrow: I. Normal erythroid development. Blood, 69, 255-263 3. Liszka, K. et al., 1983, Glycophorin A expression in malignant hematopoiesis. Am.J.Hematol., 15, 219-226 4. Villeval, J.L. et al., 1986, Phenotype of early erythroblastic leukemias. Blood, 68, 1167-1174 5. Cuneo, A., et al. 1990. Morphologic, immunologic and cytogentic studies in erythroleukemia: evidence for multilineage involvement and identification of two distinct cytogenetic-clinicopathological types. Br. J. Hematol., 75, 346-35.