Technical Data
C2549-50L
cdc2, p34
Description:
The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Applications:
Suitable for use in RIA, Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Immunomicroscopy. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen, Formalin fixed paraffin- embedded sections
Optimal dilutions to be determined by the researcher.

Controls:
Antigen standard for CDC2 (Transient overexpression lysate)

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a3C124Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceXenopusMouse
Concentration:
~0.5mg/ml
Immunogen:
C-terminal fragment starting at aa85 of Xenopus p34cdc2 expressed in E. coli. Epitope: Residues 220-227 of mouse cdc2, (LGTPNNEV) Cellular Localization: Cytoplasmic
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS. No preservatives added.
Specificity:
Recognizes Cdc2. Inhibits the activation of Cdc2, p34 kinase by cyclins. Species crossreactivity: Xenopus, human, rat, mouse, chicken, amphibian
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Kobayashi H, et al. (1991). Cyclins and their partners during Xenopus oocyte maturation. Cold Spring Harb Symp Quant Biol. 56, 437-447. 2.Kobayashi H, et al. (1992). Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits. Mol Biol Cell 3(11), 1279-1294. 3.Goodger NM, et al. (1996). The localization of p34cdc2 in the cells of normal, hyperplastic, and malignant epithelial and lymphoid tissues of the oral cavity. J Pathol. 178(4), 422-428.