Technical Data
CDC2-related Protein Kinase 7 (Cell Division Cycle 2-related Protein Kinase 7, CRK7, Cdc2-related Kinase Arginine/serine-rich, CRKR, CrkRS, Cell Division Protein Kinase 12, Cyclin-dependent Kinase 12, CDK12, hCDK12, KIAA0904)
CRK7 is a CDC2/CDK -type protein kinase. Also known as CRKRS, the protein has an arginine/serine-rich (RS) domain that is found predominantly in splicing factors, and extensive proline-rich regions that match the consensus for SH3 and WW domain binding sites. Anti-CrkRS immunoprecipitates have been shown to phosphorylate the C-terminal domain of RNA polymerase II in vitro. CRK7 has been shown to be expressed ubiquitously. Nuclear staining is seen in some carcinomas (breast, lung, others), this may be because these cells are actively dividing and this gene is associated with cell division. ESTs have been isolated from several tissue libraries, including normal lymph and cancerous bone.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 6ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Breast carcinoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to the internal domain of human CRK7.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.7, 0.01% sodium azide.
Recognizes human CRK7.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.