Technical Data
C2549-70
Cdc4 (FBXW7, F-box and WD repeat domain containing 7, AGO, Archipelago homolog, DKFZp686F23254, F-box/WD repeat-containing protein 7, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, FBW6, FBW7, FBX30, FBXO30, FBXW6, FLJ11071, FLJ16457, h
Description:
The F-box protein family is characterized by an approximately 40 amino acid motif, the F-box. F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. They constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Cdc4 is one member of this family, which is also known as AGO, FBW7, FBX30 or SEL10. CDC4 binds directly to cyclin E and targets cyclin E for ubiquitin-mediated degradation. Cdc4 proteins of amino acid lengths varying from 553 aa to 707 aa in length have been reported. Cdc4 is an inhibitor of Notch signaling that targets Notch for ubiquitin-mediated degradation after a nuclear phosphorylation event. Cdc4 interacts with presenilin 1, facilitates its ubiquitination, and alters A-beta peptide production. Thus, it may be important for Alzheimer’s disease. Mutations of the CDC4 gene are detected in ovarian and breast cancer cell lines and the CDC4 gene may also be involved in the pathogenesis of human pancreatic and endometrial cancers.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml
Immunoprecipitation: 7ug/reaction
Optimal dilutions to be determined by the researcher.

Positive Controls:
Human T47D, U2-OS, MCF-7, HepG2 and HeLa cell lysates.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to an internal region of the human and mouse Cdc4 (archipelago (AGO), F-box/WD-repeat protein 7, F-box and WD-40 domain protein 7, F-box protein FBW7, FBX30 and SEL-10). Species Sequence Homology: mouse, 100%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Cdc4 protein at ~60-80kD. Multiple bands may represent various isoforms.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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