Technical Data
C2550-05
cdc6 (CDC18L, HsCDC18, HsCDC6, p62cdc6, Cell Division Cycle 6)
Description:
The replication licensing system acts to ensure that no section of the genome is replicated more than once in a single cell cycle. The origin recognition complex (ORC) and cdc6/cdc18 are needed on chromatin before the licensing reaction can take place. During the transition from a growth-arrested to a proliferative state, transcription of mammalian cdc6 is regulated by the cell cycle transcription factor (E2F protein). Immunoblots show that quiescent cells lack cdc6 and that minichromosome maintenance (MCM) proteins are not associated with chromatin. Competence of G1 phase nuclei to replicate in vitro coincides with maximum cdc6 accumulation and MCM protein binding to chromatin in vivo. Antibodies against cdc6 and MCM5 stain abnormal (neoplastically transformed) cells in cervical smears and sections with remarkably high specificity and sensitivity. Thus antibodies against proteins that regulate DNA replication may reduce the high false-negative rate of the Pap smear test and may facilitate mass automated screening.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2 hrs. at RT.

Immunoprecipitation: 2ug/mg protein lysate (native and denatured) (Use Protein G).

Optimal dilutions to be determined by the researcher.

Hybridoma:
BALB/c splenocytes and mouse myeloma NS-2 cells.

Positive Control:
Raji or MDA231 cells.

Cellular Localization:
Nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.189Affinity Purified
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Recombinant human CDC6 protein
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2550-05X.
Specificity:
Recognizes human CDC6 at 62kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Petersen et. al. EMBO J, 1999; 18:396-410.