Technical Data
C2550-05D
CDC6 (Cell Division Control Protein 6 Homolog, CDC6-related Protein, Cdc18-related Protein, HsCdc18, p62(cdc6), HsCDC6, CDC18L)
Description:
The replication licensing system acts to ensure that no section of the genome is replicated more than once in a single cell cycle. The origin recognition complex (ORC) and CDC6/CDC18 (m.wt-62kDa) are needed on chromatin before the licensing reaction can take place. During the transition from a growth-arrested to a proliferative state, transcription of mammalian CDC6 is regulated by the cell cycle transcription factor (E2F protein). Immunoblots show that minichromosome maintenance (MCM) proteins are not associated with chromatin. Competence of G1 phase nuclei to replicate in vitro coincides with maximum CDC6 accumulation and MCM protein binding to chromatin in vivo. Antibodies against CDC6 and MCM5 stain abnormal (neoplastically transformed) cells in cervical smears and sections with remarkably high specificity and sensitivity. SOURCE AND REACTIVITY: This mouse monoclonal antibody was raised against recombinant human CDc6 protein with Ig Isotype being IgG1. Species reactivity includes human and others not tested.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 2.5-5ug/ml
Immunoprecipitation: 2ug/mg protein lysate
Optimal dilutions to be determined by the researcher.

Positive Control:
Raji or MDA231 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1SPM380Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Recombinant corresponding to human CDc6.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity:
Recognizes human CDc6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.