Technical Data
C2550-06C
Cdc6, phosphorylated (Ser54) (CDC18L, HsCDC18, HsCDC6, p62cdc6, Cell Division Cycle 6)
Description:
The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6p and cdc18+ are unstable proteins that are essential and rate limiting for the initiation of DNA replication in Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. They participate in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). Cdc6 is believed to be phosphorylated at Serine 54 during S phase and functions as a chromatin-bound signal that prevents reformation of prereplication complexes (4).

Applications:
Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E214Supernatant
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A phospho-specific peptide corresponding to residues surrounding serine 54 of human Cdc6.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human Cdc6 phosphorylated on Serine 54 at ~62kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hall JR, et al. Mol Biol Cell. 18(9):3340-50, 2007. 2. Williams RS, et al. Proc Natl Acad Sci U S A. 94(1):142-7, 1997. 3. Yan Z, et al. Proc Natl Acad Sci U S A. 95(7):3603-8, 1998 4. Jiang W, et al. Proc Natl Acad Sci U S A. 96(11):6193-8, 1999.