Technical Data
C2550-11A
CDC23/APC8 (CDC23, CDC-23, Cell division cycle 23, APC8, APC-8, Anaphase-promoting complex subunit 8)
Description:
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.

Applications:
Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000 to 1:10,000
Immunoprecipitation: 2-5ug/mg lysate
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide corresponding to a region between aa125 and 175 of human cell division cycle 23.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in tris-buffered saline, 0.1% BSA, 0.09% sodium azide.
Specificity:
Recognizes human CDC23/APC8.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.