Technical Data
C2560-01G
CDC25A, phosphorylated (Ser75) (M-phase Inducer Phosphatase 1, Dual Specificity Phosphatase Cdc25A)
Description:
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser75 of human Cdc25A (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human Cdc25A phosphorylated at Ser75.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). 2. Nemoto, K., et al., Prostate 58(1):95-102 (2004). 3. Goloudina, A., et al., Cell Cycle 2(5):473-478 (2003). 4. Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003). 5. Chow, J.P., et al., Mol. Biol. Cell 14(10):3989-4002 (2003).