Technical Data
C2560-06
CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60)
Description:
cdc25C (Mr 56 and 59kD), in asynchronous cells, is a dual specificity protein phosphatase that controls entry of cells into mitosis by dephosphorylating and activating the Cdc2/cyclin B complex and is regulated at both the transcriptional and post-translational levels. Expression of cdc25C is restricted to late S/G2, effected by a transcriptional repressor that binds the CDE (cell cycle-dependent element) in the basal promoter. cdc25C is also regulated via phosphorylation of Ser216 by C-TAK1 (cdc25c associated protein kinase) which mediates interaction with the 14-3-3 family, maintaining cdc25C “inactive”. Phosphorylated Ser216 is detectable only throughout interphase. Cells expressing cdc25C mutants that cannot be phosphorylated at Ser216 exhibit perturbed mitotic timing and escape the G2 checkpoint arrest induced by radiation induce DNA damage.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, and Western Blot. Not suitable for Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate
Western Blot: 1-2ug/ml for 2 hours at room temperature.

Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa, HT29, or LS174T cells.

Cellular Localization:
Cytoplasmic

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k0.N.192Affinity Purified
SizeStorageShippingSourceHost
500ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
200ug/ml
Immunogen:
Recombinant human cdc25C protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide.
Specificity:
Recognizes cdc25C at 53-56kD. Species Reactivity: Human. Does not react with Mouse and Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zwicker J, et al., Prog Cell Cycle Res 1995;1:91-99. 2. Sartor H, et al., Genomics 1992 Jul;13(3):911-912. 3. Taviaux SA, et al., Genomics 1993 Jan;15(1):194-196.4. Strausfeld U, et al., J Biol Chem 1994 Feb 25;269(8):5989-6000. 5. Peng CY,et al., Cell Growth Differ 1998;9(3):197-208.
6. Zwicker J, et al., Nucleic Acids Res 1995;23(19):3822-3830. 7. Korner K, et al., Nucleic Acids Res 1997 Dec 15;25(24):4933-4939. 8. Wu W,et al,. Cancer Res 1998 Sep 15;58(18):4082-4085.
9. Kudo Y, et al., Jpn J Cancer Res 1997 Oct;88(10):947-952.