Technical Data
C2572-05B
cdk 2(Cyclin-dependent Kinase 2)
Description:
Cyclin-dependent kinases (cdk) are the catalytic subunits of the cyclin/cdk complexes, which phosphorylate substrates on threonine/serine residues. Cdk2 interacts with cyclin D1, D3 and E, as well as cyclin A. Cyclin E/cdk2 kinase is active in G1- and S-phase of the cell cycle and is important for progression of cells from G1 to S. Cyclin A/cdk2 levels are maximal at the S/G2-phase transition and both are found associated with DNA in the initiation complex during replication. Rb is a substrate for cyclin E/cdk2 and cyclin A/cdk2 in vivo.

Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation: 10ul/mg; co-precipitates associated proteins
Western Blot: 1ug/ml Incubate fot 2 hours at RT.
Immunohistochemistry: 2-4ug/ml cryostat sections and paraffin embedded sections. For paraffin embedded sections, boil the sections in 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3-NS1/AG4-1 (NS1) myeloma cells with spleen cells from Balb/c mice.

Positive Control:
HeLa, 293, VA13, IMR90, Jurkat and WI-38 cells; thymus, tonsil or reactive lymph node.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b5J207Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified recombinant human cdk2 protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes human cyclin-dependent kinase-2 (cdk2) at ~33kD. Species Crosseactivity: mouse and rat. Does not cross-react with other members of the cdk family.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Akiyama, T., T. Yoshida, T. Tsujita, M. Shimizu, T. Mizukami, M. Okabe, and S.kinaga G1 phase accumulation induced by UCN-01 is associated withdephosphorylation of Rb and CDK2 proteins as well as induction of CDK inhibitorp21/Cip1/WAF1/Sdi1 in p53-mutated human epidermoid carcinoma A431 cells. CancerRes. 57(8):1495-1501.(1997)2. Bresnahan, W.A., E.A. Thompson, and T. Albrecht Human cytomegalovirusinfection results in altered Cdk2 subcellular localization. J. Gen. Virol.78(Pt 8):1993-1997.(1997)