Technical Data
Cdk2, (Cyclin-dependent Kinase 2), phosphorylated (Thr14)
Cyclin-dependent protein kinase 2 (Cdk2), also known as cell division protein kinase 2, is a 34-kD nuclear protein that regulates events within G1 and S phase in the human cell cycle (1-4). Cdk2 is inactive until a complex is formed with cyclin A, D, or E (2). Inhibitory phosphorylation occurs on Thr14 and Tyr15. Activation of the Cdk2 complex requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK (3,4).

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:5000-1:20,000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:30
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Not Determined
A synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Recognizes human Cdk2 phosphorylated on Threonine 14 at ~33kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Morgan, D.O. Nature 374: 131134 (1995). 2. Elledge, S.J., et al. Proc. Natl. Acad. Sci. USA 89: 2907-2911 (1992). 3. Gu, Y., et al. EMBO J. 11: 39954005 (1992). 4. Fesquet, D., et al. EMBO J. 12: 31113121 (1993).