Technical Data
cdk4, p34 (CDK4, Cyclin-dependent Kinase 4, p34cdk4)
Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.

Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa and K-562 cell lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20CBlue IceMouseRabbit
Synthetic peptide corresponding to the C-terminal of mouse cdk4, p34. Cellular Localization: Cytoplasmic
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Recognizes mouse Cdk4, p34. Species Crossreactivity: human and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ekholm SV & Reed SI. Curr Opin Cell Biol 12:676-84 (2000). 2. Chu CY & Lim RW. Biochim Biophys Acta 1497:175-85 (2000). 3. Morisaki H et al. Exp Cell Res 253:503-10 (1999). 4. Tong W & Pollard JW. Mol Cell Biol 19:2251-64 (1999). 5. Sweeney KJ et al. Oncogene 14:1329-40 (1997). 6. Kishimoto T & Okumura E. Prog Cell Cycle Res 3:241-9 (1997). 7. O'Connor PM et al. J Biol Chem 268:8298-308 (1993). 8. Morla AO et al. Cell 58:193-203 (1989).