		Technical Data
C2585-28	CDw49f (VLA6) (FITC)

Description:
CDw49f is expressed on epithelial and endothelial basement membrane. The VLA alpha-6 chain, which consists of a single disulfide linked 120kD subunit and a small doublet subunit of 25/30kD, is non-covalently associated with the VLA beta-chain (CD29) in VLA-6 or alpha 6/beta 1 complex or with integrin beta 4 chain (alpha 6/beta 4). The antigen is also expressed on platelets, monocytes, thymocytes and T lymphocytes.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul labels 10e6 cells
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Source:
Tissue culture supernatant

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG2b	2Q959	Affinity Purified

Size		Storage	Shipping	Source	Host
100 Tests		-20°C	Blue Ice	Human	Mouse

Concentration:

Not Determined

Immunogen:

Cell line SW/222 (Human colorectal carcinoma).

Purity:

Purified by Protein A affinity chromatography

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Specificity:

Recognizes human CDw49f.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Pignatelli, M., et al., Human Pathol. 23: 1159–1166 (1992). 2. Dobson, H., et al., GUT 35: 1343–1347 (1994). 3. Ramaswamy, A., et al., J. Path. 176(3): 227–232 (1995).