		Technical Data
C2589-57	CDX2 (CDX2, Caudal Type Homeobox Transcription Factor 2, HGNC:1806, CDX-3, CDX3)

Description:
CDX2 is a caudal type homeobox gene that encodes an intestine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa. Studies have shown that CDX2 is superior marker compared to CK20.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Paraffin): 1:25-1:50. ABC method; incubation period of 60 min at RT. FFPE tissue sections require high temperature antigen unmasking with 1mM EDTA buffer, pH 8.0 prior to immunostaining.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control: Colon cancer

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1,k	8J980	Supernatant

Size		Storage	Shipping	Source	Host
500ul		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

Not Determined

Immunogen:

Prokaryotic recombinant protein corresponding to the 180 amino acid near the N-terminal region of the human CDX2 molecule.Cellular Localization: Nuclear

Purity:

Tissue culture supernatant

Form

Supplied as a liquid, 0.1% sodium azide.

Specificity:

Recognizes human CDX2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

11. Bai YQ et al. Ectopic expression of homeodomain protein CDX2 in intestinal metaplasia and carcinomas of the stomach. Cancer Lett 176:47-55 (2002). 2. Hinoi T et al. Loss of CDX2 expression and microsatellite instability are prominent features of large cell minimally differentiated carcinomas of the colon. Am J Pathol 159:2239-48 (2001). 3. Silberg DG et al. Cdx1 and cdx2 expression during intestinal development. Gastroenterology 119:961-71 (2000).